Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions

Registros de Dados

Os dados deste recurso de evento de amostragem foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 68 registros.

Também existem 1 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.

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Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Lasa A V, Fernández González A J, Villadas P J, Fernández López M (2023). Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions. Version 2.3. Estación Experimental del Zaidín (CSIC). Samplingevent dataset. https://doi.org/10.15470/6xnz80

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é Estación Experimental del Zaidín (CSIC). This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: ffae417e-b2d8-476c-afe4-8c1093b67071.  Estación Experimental del Zaidín (CSIC) publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Spain.

Palavras-chave

Samplingevent; occurrence; Pinus pinaster; drought; bacteria; genotype; bacterial ecology

Contatos

Cobertura Geográfica

The experimental area is located in Sierra de Oria, province of Almería (Andalusia, Spain). Coordinates: 37° 31′ N2° 21′W

Cobertura Taxonômica

Plants commonly identified by people with knowledge of natural resources

Cobertura Temporal

Dados Sobre o Projeto

The project SUMHAL aims at implementing a strategy for biodiversity conservation in the Mediterranean area by setting a technologically efficient and scientifically robust system. The project combines fieldwork and virtual research environments for the recording, storing, analysis, and dissemination of the conservation status and threats of biodiversity in Andalusia (Southern Spain).

O pessoal envolvido no projeto:

Métodos de Amostragem

18 Pinus pinaster trees separated al least 50 m each other were selected in the forest of Sierra de Oria. The soil closely attached to the roots (rhizosphere) was collected. Sampling was performed on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The main roots of each tree were followed until young roots were found at a distance of less than 50 cm from the trunk (5-25 cm in depth). The soil closely attached to the roots (rhizosphere soil) was collected manually by rubbing the roots, and 2 g of each rhizosphere soil were mixed with 5 mL of LifeGuard™ Soil Preservation Solution. Samples were kept at 4 ºC until they were processed. Additional samples of soil near the roots (500 g of soil per tree) were also collected to analyze physicochemical properties, solely on July 2017. The determination of soil physicochemical parameters was performed at the Agri-Food Laboratory of the Andalusian Regional Government (Granada, Spain) under the standardized procedures developed by this service. From each rhizosphere soil sample, the total DNA and RNA were extracted by using by using the RNA PowerSoil® Total RNA Isolation Kit and the RNA PowerSoil® DNA Elution Accesory kit. The extracted RNA was retrotranscribed to double stranted cDNA. Thus, 18 samples of cDNA were obtained in summer (C17-01 to C17-18), 18 in spring (C18-01 to C18-18), and 18 samples of DNA were obtained for summer (D17-01 to D17-18) and for spring (D18-01 to D18-18).

Descrição dos passos do método:

1. All the experimental procedures were published in detail in the following research artile: "Correlating the above- and belowground genotype of Pinus pinaster trees and rhizosphere bacterial communities under drought conditions", DOI: http://dx.doi.org/10.1016/j.scitotenv.2022.155007

Metadados Adicionais