Bacterial members that comprise the rhizosphere microbiota of Pinus pinaster trees located in a forest subjected to severe drought conditions
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Pesquisadores deveriam citar esta obra da seguinte maneira:
Lasa A V, Fernández González A J, Villadas P J, Fernández López M (2022): Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions. v2.1. Estación Experimental del Zaidín (CSIC). Dataset/Samplingevent. https://doi.org/10.15470/6xnz80
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O editor e o detentor dos direitos deste trabalho é Estación Experimental del Zaidín (CSIC). This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: ffae417e-b2d8-476c-afe4-8c1093b67071. Estación Experimental del Zaidín (CSIC) publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Spain.
Samplingevent; occurrence; Pinus pinaster; drought; bacteria; genotype; bacterial ecology
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The experimental area is located in Sierra de Oria, province of Almería (Andalusia, Spain). Coordinates: 37° 31′ N2° 21′W
|Coordenadas delimitadoras||Sul Oeste [36,492, -3,274], Norte Leste [37,71, -1,285]|
Plants commonly identified by people with knowledge of natural resources
|Data Inicial / Data final||2017-07-18 / 2018-04-24|
Dados Sobre o Projeto
The project SUMHAL aims at implementing a strategy for biodiversity conservation in the Mediterranean area by setting a technologically efficient and scientifically robust system. The project combines fieldwork and virtual research environments for the recording, storing, analysis, and dissemination of the conservation status and threats of biodiversity in Andalusia (Southern Spain).
|Título||Sustainability for Mediterranean Hotspots in Andalusia integrating LifeWatch ERIC (SUMHAL). Working package 7: Improving sustainability of Mediterranean forests and silvopastoral agrosystems under climate change|
|Financiamento||This study was funded by MICINN through European Regional Development Fund [SUMHAL, LIFEWATCH-2019-09-CSIC-13, POPE 2014-2020] and by the Spanish Ministry of Economy, Industry and Competitiveness [AGL2015-66048-C2-1-R; RTI2018-098015-B-I00].|
|Descrição da Área de Estudo||The area under study in this work is located in Sierra de Oria (Almeria, Southeast Spain). This area is covered by a pine forest (dominated by Pinus pinaster and Pinus halepensis individuals) which is severly affected by intense and severe droughts. This Mediterranean area could serve as a mirror of the future climate events.|
|Descrição do Design||We aim to improve our understanding of the response of the Mediterranean forests to the main threats related to climate change. The specific aims of this working package were: 1) To explore the role of root microbiota in the decline process of Andalusian pine forests. 2) To disseminate and transfer knowledge to environmental managers and academia.|
O pessoal envolvido no projeto:
Métodos de Amostragem
18 Pinus pinaster trees separated al least 50 m each other were selected in the forest of Sierra de Oria. The soil closely attached to the roots (rhizosphere) was collected. Sampling was performed on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The main roots of each tree were followed until young roots were found at a distance of less than 50 cm from the trunk (5-25 cm in depth). The soil closely attached to the roots (rhizosphere soil) was collected manually by rubbing the roots, and 2 g of each rhizosphere soil were mixed with 5 mL of LifeGuard™ Soil Preservation Solution. Samples were kept at 4 ºC until they were processed. Additional samples of soil near the roots (500 g of soil per tree) were also collected to analyze physicochemical properties, solely on July 2017. The determination of soil physicochemical parameters was performed at the Agri-Food Laboratory of the Andalusian Regional Government (Granada, Spain) under the standardized procedures developed by this service. From each rhizosphere soil sample, the total DNA and RNA were extracted by using by using the RNA PowerSoil® Total RNA Isolation Kit and the RNA PowerSoil® DNA Elution Accesory kit. The extracted RNA was retrotranscribed to double stranted cDNA. Thus, 18 samples of cDNA were obtained in summer (C17-01 to C17-18), 18 in spring (C18-01 to C18-18), and 18 samples of DNA were obtained for summer (D17-01 to D17-18) and for spring (D18-01 to D18-18).
|Área de Estudo||The data were collected in Sierra de Oria (Almería, Southeast Spain, coordinates: 37°31′ N 2° 21′W). The place is covered by a natural population dominated by Pinus pinaster and Pinus halepensis trees, which is under the effect of severe and frequent droughts. Data were collected twice: on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The mean temperature and precipitation in June and July (2017) are 23.9 °C and 1.6 mm, and means values in March and April (2018) of 12.1 °C and 26.5 mm, respectively.|
Descrição dos passos do método:
- All the experimental procedures were published in detail in the following research artile: "Correlating the above- and belowground genotype of Pinus pinaster trees and rhizosphere bacterial communities under drought conditions", DOI: http://dx.doi.org/10.1016/j.scitotenv.2022.155007