Sampling event

Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions

最新版本 由 Estación Experimental del Zaidín (CSIC) 發佈於 2022年7月22日 Estación Experimental del Zaidín (CSIC)

下載最新版本的 Darwin Core Archive (DwC-A) 資源,或資源詮釋資料的 EML 或 RTF 文字檔。

DwC-A資料集 下載 68 紀錄 在 English 中 (6 MB) - 更新頻率: 有可能更新,但不確知何時
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說明

Bacterial members that comprise the rhizosphere microbiota of Pinus pinaster trees located in a forest subjected to severe drought conditions

資料紀錄

此資源sampling event的資料已發佈為達爾文核心集檔案(DwC-A),其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 68 筆紀錄。

亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

Event (核心)
68
Occurrence 
375496

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本,以便利追蹤其隨時間的變更。

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Lasa A V, Fernández González A J, Villadas P J, Fernández López M (2022): Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions. v2.1. Estación Experimental del Zaidín (CSIC). Dataset/Samplingevent. https://doi.org/10.15470/6xnz80

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Estación Experimental del Zaidín (CSIC)。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: ffae417e-b2d8-476c-afe4-8c1093b67071。  Estación Experimental del Zaidín (CSIC) 發佈此資源,並經由GBIF Spain同意向GBIF註冊成為資料發佈者。

關鍵字

Samplingevent; occurrence; Pinus pinaster; drought; bacteria; genotype; bacterial ecology

聯絡資訊

資源建立者:

Ana V Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES
Antonio José Fernández González
Postdoc
Estación Experimental del Zaidín
Calle Profesor Albareda 1
Granada
ES
Pablo José Villadas
Technic-Generalized graduate
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES
Manuel Fernández López
Tenured Scientist
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES

可回覆此資源相關問題者:

Ana V Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES

元數據填寫者:

Ana V Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES

與此資源的相關者:

內容提供者
Ana Vicente Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Profesor Albareda 1
Granada
ES

地理涵蓋範圍

The experimental area is located in Sierra de Oria, province of Almería (Andalusia, Spain). Coordinates: 37° 31′ N2° 21′W

界定座標範圍 緯度南界 經度西界 [36.492, -3.274], 緯度北界 經度東界 [37.71, -1.285]

分類群涵蓋範圍

Plants commonly identified by people with knowledge of natural resources

Kingdom Bacteria, Plantae

時間涵蓋範圍

起始日期 / 結束日期 2017-07-18 / 2018-04-24

計畫資料

The project SUMHAL aims at implementing a strategy for biodiversity conservation in the Mediterranean area by setting a technologically efficient and scientifically robust system. The project combines fieldwork and virtual research environments for the recording, storing, analysis, and dissemination of the conservation status and threats of biodiversity in Andalusia (Southern Spain).

計畫名稱 Sustainability for Mediterranean Hotspots in Andalusia integrating LifeWatch ERIC (SUMHAL). Working package 7: Improving sustainability of Mediterranean forests and silvopastoral agrosystems under climate change
辨識碼 LWE2021-03-025
經費來源 This study was funded by MICINN through European Regional Development Fund [SUMHAL, LIFEWATCH-2019-09-CSIC-13, POPE 2014-2020] and by the Spanish Ministry of Economy, Industry and Competitiveness [AGL2015-66048-C2-1-R; RTI2018-098015-B-I00].
研究區域描述 The area under study in this work is located in Sierra de Oria (Almeria, Southeast Spain). This area is covered by a pine forest (dominated by Pinus pinaster and Pinus halepensis individuals) which is severly affected by intense and severe droughts. This Mediterranean area could serve as a mirror of the future climate events.
研究設計描述 We aim to improve our understanding of the response of the Mediterranean forests to the main threats related to climate change. The specific aims of this working package were: 1) To explore the role of root microbiota in the decline process of Andalusian pine forests. 2) To disseminate and transfer knowledge to environmental managers and academia.

參與計畫的人員:

研究主持人
Manuel Fernández López
Ana V Lasa
作者
Antonio José Fernández González
作者
Pablo José Villadas Latorre

取樣方法

18 Pinus pinaster trees separated al least 50 m each other were selected in the forest of Sierra de Oria. The soil closely attached to the roots (rhizosphere) was collected. Sampling was performed on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The main roots of each tree were followed until young roots were found at a distance of less than 50 cm from the trunk (5-25 cm in depth). The soil closely attached to the roots (rhizosphere soil) was collected manually by rubbing the roots, and 2 g of each rhizosphere soil were mixed with 5 mL of LifeGuard™ Soil Preservation Solution. Samples were kept at 4 ºC until they were processed. Additional samples of soil near the roots (500 g of soil per tree) were also collected to analyze physicochemical properties, solely on July 2017. The determination of soil physicochemical parameters was performed at the Agri-Food Laboratory of the Andalusian Regional Government (Granada, Spain) under the standardized procedures developed by this service. From each rhizosphere soil sample, the total DNA and RNA were extracted by using by using the RNA PowerSoil® Total RNA Isolation Kit and the RNA PowerSoil® DNA Elution Accesory kit. The extracted RNA was retrotranscribed to double stranted cDNA. Thus, 18 samples of cDNA were obtained in summer (C17-01 to C17-18), 18 in spring (C18-01 to C18-18), and 18 samples of DNA were obtained for summer (D17-01 to D17-18) and for spring (D18-01 to D18-18).

研究範圍 The data were collected in Sierra de Oria (Almería, Southeast Spain, coordinates: 37°31′ N 2° 21′W). The place is covered by a natural population dominated by Pinus pinaster and Pinus halepensis trees, which is under the effect of severe and frequent droughts. Data were collected twice: on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The mean temperature and precipitation in June and July (2017) are 23.9 °C and 1.6 mm, and means values in March and April (2018) of 12.1 °C and 26.5 mm, respectively.

方法步驟描述:

  1. All the experimental procedures were published in detail in the following research artile: "Correlating the above- and belowground genotype of Pinus pinaster trees and rhizosphere bacterial communities under drought conditions", DOI: http://dx.doi.org/10.1016/j.scitotenv.2022.155007

額外的詮釋資料

替代的識別碼 10.15470/6xnz80
ffae417e-b2d8-476c-afe4-8c1093b67071
https://ipt.gbif.es/resource?r=pinuspinaster_genotype