Descripción
Bacterial members that comprise the rhizosphere microbiota of Pinus pinaster trees located in a forest subjected to severe drought conditions
Registros
Los datos en este recurso de evento de muestreo han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 68 registros.
también existen 1 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Lasa A V, Fernández González A J, Villadas P J, Fernández López M (2023). Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions. Version 2.3. Estación Experimental del Zaidín (CSIC). Samplingevent dataset. https://doi.org/10.15470/6xnz80
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es Estación Experimental del Zaidín (CSIC). Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: ffae417e-b2d8-476c-afe4-8c1093b67071. Estación Experimental del Zaidín (CSIC) publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Spain.
Palabras clave
Samplingevent; occurrence; Pinus pinaster; drought; bacteria; genotype; bacterial ecology
Contactos
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
- Originador
- Originador
- Originador
- Proveedor De Contenido
Cobertura geográfica
The experimental area is located in Sierra de Oria, province of Almería (Andalusia, Spain). Coordinates: 37° 31′ N2° 21′W
| Coordenadas límite | Latitud Mínima Longitud Mínima [36,492, -3,274], Latitud Máxima Longitud Máxima [37,71, -1,285] |
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Cobertura taxonómica
Plants commonly identified by people with knowledge of natural resources
| Reino | Bacteria, Plantae |
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Cobertura temporal
| Fecha Inicial / Fecha Final | 2017-07-18 / 2018-04-24 |
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Datos del proyecto
The project SUMHAL aims at implementing a strategy for biodiversity conservation in the Mediterranean area by setting a technologically efficient and scientifically robust system. The project combines fieldwork and virtual research environments for the recording, storing, analysis, and dissemination of the conservation status and threats of biodiversity in Andalusia (Southern Spain).
| Título | Sustainability for Mediterranean Hotspots in Andalusia integrating LifeWatch ERIC (SUMHAL). Working package 7: Improving sustainability of Mediterranean forests and silvopastoral agrosystems under climate change |
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| Identificador | LIFEWATCH-2019-09-CSIC-4, POPE 2014-2020 |
| Fuentes de Financiación | This study was funded by MICINN through European Regional Development Fund [SUMHAL, LIFEWATCH-2019-09-CSIC-4, POPE 2014-2020] and by the Spanish Ministry of Economy, Industry and Competitiveness [AGL2015-66048-C2-1-R; RTI2018-098015-B-I00]. To be referred from 2023 onwards as SUMHAL, LIFEWATCH-2019-09-CSIC-4, POPE 2014-2020. |
| Descripción del área de estudio | The area under study in this work is located in Sierra de Oria (Almeria, Southeast Spain). This area is covered by a pine forest (dominated by Pinus pinaster and Pinus halepensis individuals) which is severly affected by intense and severe droughts. This Mediterranean area could serve as a mirror of the future climate events. |
| Descripción del diseño | We aim to improve our understanding of the response of the Mediterranean forests to the main threats related to climate change. The specific aims of this working package were: 1) To explore the role of root microbiota in the decline process of Andalusian pine forests. 2) To disseminate and transfer knowledge to environmental managers and academia. |
Personas asociadas al proyecto:
- Investigador Principal
- Autor
- Autor
Métodos de muestreo
18 Pinus pinaster trees separated al least 50 m each other were selected in the forest of Sierra de Oria. The soil closely attached to the roots (rhizosphere) was collected. Sampling was performed on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The main roots of each tree were followed until young roots were found at a distance of less than 50 cm from the trunk (5-25 cm in depth). The soil closely attached to the roots (rhizosphere soil) was collected manually by rubbing the roots, and 2 g of each rhizosphere soil were mixed with 5 mL of LifeGuard™ Soil Preservation Solution. Samples were kept at 4 ºC until they were processed. Additional samples of soil near the roots (500 g of soil per tree) were also collected to analyze physicochemical properties, solely on July 2017. The determination of soil physicochemical parameters was performed at the Agri-Food Laboratory of the Andalusian Regional Government (Granada, Spain) under the standardized procedures developed by this service. From each rhizosphere soil sample, the total DNA and RNA were extracted by using by using the RNA PowerSoil® Total RNA Isolation Kit and the RNA PowerSoil® DNA Elution Accesory kit. The extracted RNA was retrotranscribed to double stranted cDNA. Thus, 18 samples of cDNA were obtained in summer (C17-01 to C17-18), 18 in spring (C18-01 to C18-18), and 18 samples of DNA were obtained for summer (D17-01 to D17-18) and for spring (D18-01 to D18-18).
| Área de Estudio | The data were collected in Sierra de Oria (Almería, Southeast Spain, coordinates: 37°31′ N 2° 21′W). The place is covered by a natural population dominated by Pinus pinaster and Pinus halepensis trees, which is under the effect of severe and frequent droughts. Data were collected twice: on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The mean temperature and precipitation in June and July (2017) are 23.9 °C and 1.6 mm, and means values in March and April (2018) of 12.1 °C and 26.5 mm, respectively. |
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Descripción de la metodología paso a paso:
- All the experimental procedures were published in detail in the following research artile: "Correlating the above- and belowground genotype of Pinus pinaster trees and rhizosphere bacterial communities under drought conditions", DOI: http://dx.doi.org/10.1016/j.scitotenv.2022.155007
Metadatos adicionales
| Identificadores alternativos | 10.15470/6xnz80 |
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| ffae417e-b2d8-476c-afe4-8c1093b67071 | |
| https://ipt.gbif.es/resource?r=pinuspinaster_genotype |