Données d'échantillonnage

Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions

Dernière version Publié par Estación Experimental del Zaidín (CSIC) le 22 juillet 2022 Estación Experimental del Zaidín (CSIC)

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Description

Bacterial members that comprise the rhizosphere microbiota of Pinus pinaster trees located in a forest subjected to severe drought conditions

Enregistrements de données

Les données de cette ressource données d'échantillonnage ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 68 enregistrements.

1 tableurs de données d'extension existent également. Un enregistrement d'extension fournit des informations supplémentaires sur un enregistrement du cœur de standard (core). Le nombre d'enregistrements dans chaque tableur de données d'extension est illustré ci-dessous.

Event (noyau)
68
Occurrence 
375496

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Versions

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Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Lasa A V, Fernández González A J, Villadas P J, Fernández López M (2022): Bacterial members of the Pinus pinaster rhizosphere microbiota in a forest subjected to drought conditions. v2.1. Estación Experimental del Zaidín (CSIC). Dataset/Samplingevent. https://doi.org/10.15470/6xnz80

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est Estación Experimental del Zaidín (CSIC). Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : ffae417e-b2d8-476c-afe4-8c1093b67071.  Estación Experimental del Zaidín (CSIC) publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Spain.

Mots-clé

Samplingevent; occurrence; Pinus pinaster; drought; bacteria; genotype; bacterial ecology

Contacts

Personne ayant créé cette ressource:

Ana V Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES
Antonio José Fernández González
Postdoc
Estación Experimental del Zaidín
Calle Profesor Albareda 1
Granada
ES
Pablo José Villadas
Technic-Generalized graduate
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES
Manuel Fernández López
Tenured Scientist
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES

Personne pouvant répondre aux questions sur la ressource:

Ana V Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES

Personne ayant renseigné les métadonnées:

Ana V Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Calle Profesor Albareda 1
Granada
ES

Autres personnes associées à la ressource:

Fournisseur de Contenu
Ana Vicente Lasa
Postdoc
Estación Experimental del Zaidín (CSIC)
Profesor Albareda 1
Granada
ES

Couverture géographique

The experimental area is located in Sierra de Oria, province of Almería (Andalusia, Spain). Coordinates: 37° 31′ N2° 21′W

Enveloppe géographique Sud Ouest [36,492, -3,274], Nord Est [37,71, -1,285]

Couverture taxonomique

Plants commonly identified by people with knowledge of natural resources

Kingdom Bacteria, Plantae

Couverture temporelle

Date de début / Date de fin 2017-07-18 / 2018-04-24

Données sur le projet

The project SUMHAL aims at implementing a strategy for biodiversity conservation in the Mediterranean area by setting a technologically efficient and scientifically robust system. The project combines fieldwork and virtual research environments for the recording, storing, analysis, and dissemination of the conservation status and threats of biodiversity in Andalusia (Southern Spain).

Titre Sustainability for Mediterranean Hotspots in Andalusia integrating LifeWatch ERIC (SUMHAL). Working package 7: Improving sustainability of Mediterranean forests and silvopastoral agrosystems under climate change
Identifiant LWE2021-03-025
Financement This study was funded by MICINN through European Regional Development Fund [SUMHAL, LIFEWATCH-2019-09-CSIC-13, POPE 2014-2020] and by the Spanish Ministry of Economy, Industry and Competitiveness [AGL2015-66048-C2-1-R; RTI2018-098015-B-I00].
Description du domaine d'étude / de recherche The area under study in this work is located in Sierra de Oria (Almeria, Southeast Spain). This area is covered by a pine forest (dominated by Pinus pinaster and Pinus halepensis individuals) which is severly affected by intense and severe droughts. This Mediterranean area could serve as a mirror of the future climate events.
Description du design We aim to improve our understanding of the response of the Mediterranean forests to the main threats related to climate change. The specific aims of this working package were: 1) To explore the role of root microbiota in the decline process of Andalusian pine forests. 2) To disseminate and transfer knowledge to environmental managers and academia.

Les personnes impliquées dans le projet:

Chercheur Principal
Manuel Fernández López
Ana V Lasa
Auteur
Antonio José Fernández González
Auteur
Pablo José Villadas Latorre

Méthodes d'échantillonnage

18 Pinus pinaster trees separated al least 50 m each other were selected in the forest of Sierra de Oria. The soil closely attached to the roots (rhizosphere) was collected. Sampling was performed on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The main roots of each tree were followed until young roots were found at a distance of less than 50 cm from the trunk (5-25 cm in depth). The soil closely attached to the roots (rhizosphere soil) was collected manually by rubbing the roots, and 2 g of each rhizosphere soil were mixed with 5 mL of LifeGuard™ Soil Preservation Solution. Samples were kept at 4 ºC until they were processed. Additional samples of soil near the roots (500 g of soil per tree) were also collected to analyze physicochemical properties, solely on July 2017. The determination of soil physicochemical parameters was performed at the Agri-Food Laboratory of the Andalusian Regional Government (Granada, Spain) under the standardized procedures developed by this service. From each rhizosphere soil sample, the total DNA and RNA were extracted by using by using the RNA PowerSoil® Total RNA Isolation Kit and the RNA PowerSoil® DNA Elution Accesory kit. The extracted RNA was retrotranscribed to double stranted cDNA. Thus, 18 samples of cDNA were obtained in summer (C17-01 to C17-18), 18 in spring (C18-01 to C18-18), and 18 samples of DNA were obtained for summer (D17-01 to D17-18) and for spring (D18-01 to D18-18).

Etendue de l'étude The data were collected in Sierra de Oria (Almería, Southeast Spain, coordinates: 37°31′ N 2° 21′W). The place is covered by a natural population dominated by Pinus pinaster and Pinus halepensis trees, which is under the effect of severe and frequent droughts. Data were collected twice: on July 18, 2017 (summer samples) and on April 24, 2018 (spring samples). The mean temperature and precipitation in June and July (2017) are 23.9 °C and 1.6 mm, and means values in March and April (2018) of 12.1 °C and 26.5 mm, respectively.

Description des étapes de la méthode:

  1. All the experimental procedures were published in detail in the following research artile: "Correlating the above- and belowground genotype of Pinus pinaster trees and rhizosphere bacterial communities under drought conditions", DOI: http://dx.doi.org/10.1016/j.scitotenv.2022.155007

Métadonnées additionnelles

Identifiants alternatifs 10.15470/6xnz80
ffae417e-b2d8-476c-afe4-8c1093b67071
https://ipt.gbif.es/resource?r=pinuspinaster_genotype