Banco de Germoplasma de la Universidad Rey Juan Carlos

Última versión Publicado por Universidad Rey Juan Carlos en Nov 30, 2018 Universidad Rey Juan Carlos

El Banco de Germoplasma de la URJC se fundó en 2008 y actualmente consta de 392 accesiones y 191 especies. Está basado en la conservación de comunidades de plantas silvestres. El objetivo del banco es conservar ex situ una muestra representativa de toda la biodiversidad vegetal característica de un hábitat, haciendo énfasis en aquellos ecosistemas definidos como prioritarios por la Directiva de Hábitats. Además, se utiliza para almacenar material con fines investigadores y docentes. Está compuesto por dos subcolecciones típicas del centro peninsular: alta montaña (pastos psicroxerófilos) y semiárida (estepas gipsófilas), una subcolección representativa del género Lupinus y otra del género Cheirolophus procedente del archipiélago Canario. Todas las accesiones se conservan en tarros herméticos rellenos de silicagel guardados en congelador a -18ºC. The Germplasm Bank of the URJC was created in 2008 and presently withholds 392 accessions and 191 species. It is based on the conservation of wild-plant communities. The aim of this bank is to conserve ex situ a representative sample of the plant biodiversity present in a habitat, putting an emphasis on those ecosystems identified as prioritary by the Habitats Directive. Moreover, it is used to store plant material for research and teaching purposes. It consists of two subcollections corresponding to habitats typical of the center of the Iberian peninsula: high mountain (psicroxerophylous pastures) and semi-arid (gypsophylic steppes), a representative subcollection of genus Lupinus and other of genus Cheirolophus from Canary Islands. All accesions are stored in the freezer at -18ºC in Kilner jars with silicagel.

Registros

Los datos en este registros biológicos recurso han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 371 registros.

Este IPT archiva los datos, sirviendo así como repositorio de datos. Los datos y metadatos están disponibles para descargar en la sección de descargas. La tabla de versiones muestra otras versiones del recurso que se han hecho accesibles al público y permite el seguimiento de los cambios hechos al recurso en el tiempo.

Descargas

Descargue la última versión de los datos como un Archivo Darwin Core (DwC-A) o los metadatos como EML o RTF:

Datos como un archivo DwC-A descargar 371 registros en Inglés (27 KB) - Frecuencia de actualización: diario
Metadatos como un archivo EML descargar en Inglés (23 KB)
Metadatos como un archivo RTF descargar en Inglés (22 KB)

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Universidad Rey Juan Carlos (2013 -). URJC GB dataset: Community-based seed bank of Mediterranean high-mountain and semi-arid plant species at Universidad Rey Juan Carlos (Spain), 235 data records, Online, http://www.gbif.es:8080/ipt/resource.do?r=germoplasma-urjc, Version 3.0 (last updated on 02/08/2018)

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es Universidad Rey Juan Carlos. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 59bf2c83-1e3c-40c8-9437-39ce3d3d462c.  Universidad Rey Juan Carlos publica este recurso, y está registrado en GBIF como un publicador de datos avalado por GBIF Spain.

Palabras Clave

accesion; germplasm bank; gypsum; high mountain; Madrid; seeds; seedbank; semi-arid; Spain; Universidad Rey Juan Carlos; wild species; Occurrence

Datos externos

Los datos del recurso también están disponibles en otros formatos

NAhttp://NA utf-8 Darwin Core Archive 1.0

Contactos

¿Quién creó el recurso?:

Patricia Alonso Valiente
Técnico de la colección
Universidad Rey Juan Carlos Tulipán s/n 28933 Móstoles Madrid ES +34 91 488 8250

¿Quién puede resolver dudas acerca del recurso?:

José María Iriondo Alegría
Investigador principal
Universidad Rey Juan Carlos Tulipán s/n 28933 Móstoles Madrid ES +34 91 488 8144
http://iriondo.weebly.com/

¿Quién documentó los metadatos?:

Patricia Alonso Valiente
Técnico de la colección
Universidad Rey Juan Carlos Tulipán s/n 28933 Móstoles Madrid ES +34 91 488 8250

¿Quién más está asociado con el recurso?:

Investigador Principal
José María Iriondo Alegría
Investigador principal
Universidad Rey Juan Carlos Tulipán s/n 28933 Móstoles Madrid ES +34 91 488 8144
http://iriondo.weebly.com/

Cobertura Geográfica

High-mountain pastures (psicroxerophylous pastures) and semi-arid habitats (gypsophylic steppes) in the center of the Iberian Peninsula.

Coordenadas límite Latitud Mínima Longitud Mínima [27.42, -20.49], Latitud Máxima Longitud Máxima [45.951, 18.721]

Cobertura Taxonómica

The URJC Germplasm Bank holds the seeds of vascular plants from specific habitats. The high-mountain subcollection is composed of communities of siliceous psicroxerophilous pastures dominated by Festuca curvifolia and rich in hemicryptophytes and chamaephytes. The semi-arid subcollection is composed of gypsophytes characteristic of semi-arid environments dominated by Cistaceae, Asteraceae and Labiatae. The Lupinus subcollection is composed of seeds from different species in this genus collected in different regions in Spain. Finally, the Canary subcollection is composed of different species of genus Cheirolophus.

Reino  Plantae
Filo  Magnoliophyta, Pinophyta
Class  Liliopsida, Magnoliposida, Pinopsida
Orden  Apiales, Asparagales, Asterales, Boraginales, Brassicales, Caryophyllales, Cupressales, Ericales, Fabales, Gentianales, Lamiales, Liliales, Malpighiales, Malvales, Poales, Ranunculales, Rosales, Saxifragales
Familia  Compositae, Labiatae, Cistaceae, Gramineae, Caryophyllaceae, Cruciferae, Leguminosae, Scrophulariaceae, Campanulaceae, Plumbaginaceae, Juncaceae, Liliaceae, Ranunculaceae, Crassulaceae, Cupressaceae, Iridaceae, Linaceae, Plantaginaceae, Polygonaceae, Rosaceae, Saxifragaceae, Amaryllidaceae, Boraginaceae, Gentianaceae, Primulaceae, Umbelliferae, Violaceae

Cobertura Temporal

Fecha Inicial / Fecha Final 2001-01-01 / 2015-01-01

Datos del Proyecto

No hay descripción disponible

Título URJC GB Dataset: Community-based seed bank of Mediterranean high mountain and semi-arid plant species at Universidad Rey Juan Carlos.
Fuentes de Financiación This project is financed by LIMITES (CGL2009-07229) and AdAptA(CGL2012-33528) research projects of the Spanish Ministry of Science and Innovation and Remedinal-2 project of the Autonomous Community of Madrid.
Descripción del Área de Estudio High mountain Mediterranean systems and semi-arid Mediterranean ecosystems.
Descripción del Diseño Collection and conservation of seeds of plant species representative of the mentioned ecosystems.

Personas asociadas al proyecto:

Investigador Principal
José María Iriondo

Métodos de Muestreo

In the collection phase, seed samples were taken from several different localities to cover the distribution range of the communities in the study area and thereby obtain genetic material from the different species with the potential to adapt to different local conditions. To plan seed collection, it was essential to determine the timing of seed dispersal. In this sense, the literature related to the phenology of the collected species was consulted. Other morphological indicators of the timing of natural dispersal were also used. These included tissue hardness, changes in color in seeds and fruits, dryness of pods and capsules, among others. The physical quality of the seeds was evaluated at the time of collection to avoid collecting specimens that were infected or clearly unviable. We aimed to collect between 3000 and 5000 seeds per accession to guarantee that there is enough material for germination assays, long-term conservation and, if necessary, propagation (Gold et al. 2004). In order not to compromise the viability of the population in situ, no more than 20% of the seeds present at the time of collection were sampled (Gold et al. 2004). Once samples were collected for each species, they were placed in paper bags and labeled with the name of the species, the site where they were collected, altitude, site coordinates, the date of collection and the name of the collector. This information constituted the passport data for each seed accession.

Área de Estudio The Sierra de Guadarrama and gypsophylic steppes of Central Spain are the most significant areas represented in URJC Germplasm Bank. Seeds have been collected from 8 provinces of Spain: Ávila, Cuenca, Guadalajara, Madrid, Salamanca, Segovia, Valencia y Zaragoza.
Control de Calidad The URJC Germplasm Bank has a protocol which describes the seed processing methods for collection, processing in the laboratory, germination assays, dessication, recording accessions, labeling, scanning and freezing. Seed collectors are professors and researchers with great knowledge of the flora characteristic of each ecosystem. They are all members of research groups that have worked in this field for years and, therefore, have great experience in the phenology and identification of the species. Moreover, some accessions have herbarium specimens. 63% of the records are georreferenced with GPS and radio points. When this information is not available, the geographic coordinates of the site and the extension of the municipality are used as a surrogate of the measure.

Descripción de la metodología paso a paso:

  1. Seed processing in the laboratory: The first treatment consisted of cleaning the seeds. Sieves with stainless steel meshes of different sizes (mesh holes between 2 mm-0.5 cm) were used to separate as much undesired material as possible (soil, stones, small leaves, stems, flowers, etc.) from the seed sample. The separated seeds were then introduced in tubes identified with the passport data (species name, date, site, altitude, collector) and stored in a cupboard at room temperature under ambient moisture conditions. Germination assays: Germination assays were carried out with 100 seeds of each accession. Four replicates of twenty-five seeds were placed in Petri dishes on two pieces of filter paper. Distilled water was added until the surface was wet and the dishes were placed in a germination chamber (Selecta Hotcold GL, Barcelona, Spain). Accessions from the high mountain collection were incubated at 15ºC with a 16/8 hour light/dark photoperiod (Giménez-Benavides et al. 2005), while accessions from the semi-arid collection were incubated at 20ºC with the same photoperiod (Pérez-García and Durán 1989, Escudero et al. 1997, Herranz et al. 2002). Assays were monitored every 2-3 days for a minimum period of one month, and the assay was considered to have finished when no germination was observed for four subsequent censuses (8-day period). Germinated seeds were counted and removed in each census. Dessication: Silica gel (SiO2) was used to desiccate the samples. As the desiccation rate increases with the amount of gel used, the base of the desiccator was filled to a 1:1 proportion with the seeds. The seeds which were separated by accessions in the Petri dishes were placed uncovered on the metal plate in the desiccator. They were then placed in a cool place to dry for at least two months. Recording accessions: Accessions were added to the database of the URJC Germplasm Bank using Herbar Zoorbar Ligero (HZL) software. Each accession is assigned a reference number allocated consecutively. The information contained in the record of each accession is: dataset, institution, catalogue number, scientific name, family, genus, specific epithet, taxon rank, infraspecific epithet, scientific name authorship, collection code, number of mother plants, number of tubes (in which the accession is stored), number of seeds, seed weight, community name, country, country code, state province, municipality, locality, decimal latitude, decimal longitude, geodetic datum, coordinate uncertainty in meters, elevation, collector, number of jar (in which the accession is stored), number of drawer (in which the accession is stored), collection date, sample acquisition date, % germination and observations. All data are standardized by DarwinCore 1.2 and have been validated by Darwin Test. We generated metadata with DarwinCore Archive in order to publish data in GBIF IPT. Labelling: After registering the accession in the database, seed accessions were labelled for proper identification. Scanning: Approximately 50 seeds were uniformly distributed avoiding contact between them and scanned. A resolution of 300 ppp was used, except in the case of very small seeds when 600 ppp resolution was used. Freezing: All of the collections in the URJC Germplasm Bank are base collections; i.e. they are stored for long periods of time and are only used in regeneration processes. Storage is carried out under optimal conditions to maximize seed viability. The procedure followed at the URJC Germplasm Bank was to store seeds in glass tubes in Kilner jars with silica gel. These jars were sealed hermetically and placed in a freezer at -18ºC.

Datos de la Colección

Nombre de la Colección Banco de Germoplasma de la Universidad Rey Juan Carlos
Identificador de la Colección http://www.gbif.es:8080/ipt/manage/metadata-citations.do?r=germoplasma-urjc
Identificador de la Colección Parental NA
Métodos de preservación de los ejemplares Congelado

Referencias Bibliográficas

  1. Escudero A, Carnes LF, Pérez-García (1997) Seed germination of gypsophytes and gypsovags in semi-arid central Spain. Journal of Arid Environments 36: 487-497.
  2. European Community (1992) Council Directive 92/43/EEC on the conservation of natural habitats and of wild fauna and flora.
  3. Fernández-González F (1987) Estudio florístico y fitosociológico del Valle del Paular (Madrid). PhD Thesis, Universidad Complutense de Madrid, Madrid.
  4. Ferrandis P, Herranz JM, Copete MA (2005) Caracterización florística y edáfica de las estepas yesosas de Castilla-La Mancha. Investigación Agraria: Sistemas y Recursos Forestales 14: 195-216.
  5. García-Romero A, Muñoz-Jiménez J (2010) Modificaciones recientes de la cubierta nival y evolución de la vegetación supraforestal en la Sierra de Guadarrama, España: el Puerto de los Neveros. Cuadernos de Investigación Geográfica nº 36 (2): 107-141.
  6. Giménez-Benavides L, Escudero A, Pérez-García F (2005). Seed germination of high mountain Mediterranean species: altitudinal, interpopulation and interannual variability. Ecological Research 20: 433-444. doi: 10.1007/s11284-005-0059-4
  7. Giménez-Benavides L (2006) Cambio climático en la alta montaña mediterránea. Ecología reproductiva, potencial adaptativo y viabilidad poblacional de Silene ciliata. PhD Thesis, Móstoles, Madrid.
  8. Giménez-Benavides L, Escudero A, Iriondo JM (2007) Reproductive limits of a late-flowering high mountain Mediterranean plant along an elevational climate gradient. New Phytologist 173: 367-382. doi: 10.1111/j.1469-8137.2006.01932.x
  9. Gold K, León-Lobos P, Way M (2004) Manual de recolección de semillas de plantas silvestres para conservación a largo plazo y restauración ecológica. Boletín INIA nº 110 (62 p.). Centro Regional de Investigación Intihuasi, Instituto de Investigación Agropecuarias, La Serena, Chile.
  10. Gómez-Campo C (1985) The Conservation of Mediterranean Plants: Principles and Problems. In: Gómez-Campo C (Ed) Plant Conservation in the Mediterranean Area. W Junk Publishers, Dordrecht, Holland, 269 pp.
  11. Herranz JM, Ferrandis P Copete MA, Martínez-Sánchez JJ (2002) Influencia de la temperatura de incubación sobre la germinación de veintitrés endemismos vegetales ibéricos e iberoafricanos. Invest. Agr.: Prod. Prot. Veg. Vol 17 (2).
  12. Martín-Herrero J, Cirujano S, Moreno M, Peris J, Stübing G (2003) La vegetación protegida de Castilla-La Mancha. Descripción, ecología y conservación de los hábitat de protección especial. Dirección General del medio Natural, Conserjería de Medio Ambiente y Junta de Comunidades de Castilla La Mancha, Toledo.
  13. Pérez-García F, Durán JM (1989) Germinación de especies endémicas de las regiones mediterránea occidental y macaronésica. Investigación Agraria: Producción y Protección vegetal 4 (1).
  14. Prance GT (1997) The Conservation of Botanical Diversity. In: Maxted N, Ford-Lloyd BV, Hawkes JG (Eds) Plant Genetic Conservation. The In Situ Approach: 3-14. Chapman & Hall, London.
  15. Rivas Martínez S (1963) Estudio de la vegetación y flora de la Sierra de Guadarrama y Gredos. Anales Jard. Bot. Madrid 21 (2): 5-325.
  16. Vielva JA, Granados I, Prieto D (2004) Restauración de ecosistemas de montaña. El Parque Natural de Peñalara. Boletín de la Real Sociedad Española de Historia Natural (sección Geológica) 99 (1-4): 209-216.
  17. Vitales D, García-Fernández A, Pellicer J, Vallés J, Santos-Guerra A et al. (2014). Key processes for Cheirolophus (Asteraceae). Diversification on oceanic islands inferred from AFLP data. PLOS One 9(11): e113207. doi:10.1371/journal.pone.0113207

Metadatos Adicionales

Identificadores Alternativos 59bf2c83-1e3c-40c8-9437-39ce3d3d462c
doi:10.15468/wtjkzk
https://ipt.gbif.es/resource?r=germoplasma-urjc