Banco de Germoplasma de la Universidad Rey Juan Carlos

Enregistrements de données

Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 495 enregistrements.

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Versions

Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Universidad Rey Juan Carlos (2013 -). URJC GB dataset: Community-based seed bank of Mediterranean high-mountain and semi-arid plant species at Universidad Rey Juan Carlos (Spain), 235 data records, Online, http://www.gbif.es:8080/ipt/resource.do?r=germoplasma-urjc, Version 3.0 (last updated on 02/08/2018)

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est Universidad Rey Juan Carlos. Ce travail est sous licence Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 59bf2c83-1e3c-40c8-9437-39ce3d3d462c.  Universidad Rey Juan Carlos publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Spain.

Mots-clé

accesion; germplasm bank; gypsum; high mountain; Madrid; seeds; seedbank; semi-arid; Spain; Universidad Rey Juan Carlos; wild species; Occurrence; Occurrence

Données externes

Les données de la ressource sont disponibles dans d'autres formats

Contacts

Couverture géographique

High-mountain pastures (psicroxerophylous pastures) and semi-arid habitats (gypsophylic steppes) in the center of the Iberian Peninsula.

Couverture taxonomique

The URJC Germplasm Bank holds the seeds of vascular plants from specific habitats. The high-mountain subcollection is composed of communities of siliceous psicroxerophilous pastures dominated by Festuca curvifolia and rich in hemicryptophytes and chamaephytes. The semi-arid subcollection is composed of gypsophytes characteristic of semi-arid environments dominated by Cistaceae, Asteraceae and Labiatae. The Lupinus subcollection is composed of seeds from different species in this genus collected in different regions in Spain. Finally, the Canary subcollection is composed of different species of genus Cheirolophus.

Couverture temporelle

Données sur le projet

Pas de description disponible

Les personnes impliquées dans le projet:

Méthodes d'échantillonnage

In the collection phase, seed samples were taken from several different localities to cover the distribution range of the communities in the study area and thereby obtain genetic material from the different species with the potential to adapt to different local conditions. To plan seed collection, it was essential to determine the timing of seed dispersal. In this sense, the literature related to the phenology of the collected species was consulted. Other morphological indicators of the timing of natural dispersal were also used. These included tissue hardness, changes in color in seeds and fruits, dryness of pods and capsules, among others. The physical quality of the seeds was evaluated at the time of collection to avoid collecting specimens that were infected or clearly unviable. We aimed to collect between 3000 and 5000 seeds per accession to guarantee that there is enough material for germination assays, long-term conservation and, if necessary, propagation (Gold et al. 2004). In order not to compromise the viability of the population in situ, no more than 20% of the seeds present at the time of collection were sampled (Gold et al. 2004). Once samples were collected for each species, they were placed in paper bags and labeled with the name of the species, the site where they were collected, altitude, site coordinates, the date of collection and the name of the collector. This information constituted the passport data for each seed accession.

Description des étapes de la méthode:

1. Seed processing in the laboratory: The first treatment consisted of cleaning the seeds. Sieves with stainless steel meshes of different sizes (mesh holes between 2 mm-0.5 cm) were used to separate as much undesired material as possible (soil, stones, small leaves, stems, flowers, etc.) from the seed sample. The separated seeds were then introduced in tubes identified with the passport data (species name, date, site, altitude, collector) and stored in a cupboard at room temperature under ambient moisture conditions. Germination assays: Germination assays were carried out with 100 seeds of each accession. Four replicates of twenty-five seeds were placed in Petri dishes on two pieces of filter paper. Distilled water was added until the surface was wet and the dishes were placed in a germination chamber (Selecta Hotcold GL, Barcelona, Spain). Accessions from the high mountain collection were incubated at 15ºC with a 16/8 hour light/dark photoperiod (Giménez-Benavides et al. 2005), while accessions from the semi-arid collection were incubated at 20ºC with the same photoperiod (Pérez-García and Durán 1989, Escudero et al. 1997, Herranz et al. 2002). Assays were monitored every 2-3 days for a minimum period of one month, and the assay was considered to have finished when no germination was observed for four subsequent censuses (8-day period). Germinated seeds were counted and removed in each census. Dessication: Silica gel (SiO2) was used to desiccate the samples. As the desiccation rate increases with the amount of gel used, the base of the desiccator was filled to a 1:1 proportion with the seeds. The seeds which were separated by accessions in the Petri dishes were placed uncovered on the metal plate in the desiccator. They were then placed in a cool place to dry for at least two months. Recording accessions: Accessions were added to the database of the URJC Germplasm Bank using Herbar Zoorbar Ligero (HZL) software. Each accession is assigned a reference number allocated consecutively. The information contained in the record of each accession is: dataset, institution, catalogue number, scientific name, family, genus, specific epithet, taxon rank, infraspecific epithet, scientific name authorship, collection code, number of mother plants, number of tubes (in which the accession is stored), number of seeds, seed weight, community name, country, country code, state province, municipality, locality, decimal latitude, decimal longitude, geodetic datum, coordinate uncertainty in meters, elevation, collector, number of jar (in which the accession is stored), number of drawer (in which the accession is stored), collection date, sample acquisition date, % germination and observations. All data are standardized by DarwinCore 1.2 and have been validated by Darwin Test. We generated metadata with DarwinCore Archive in order to publish data in GBIF IPT. Labelling: After registering the accession in the database, seed accessions were labelled for proper identification. Scanning: Approximately 50 seeds were uniformly distributed avoiding contact between them and scanned. A resolution of 300 ppp was used, except in the case of very small seeds when 600 ppp resolution was used. Freezing: All of the collections in the URJC Germplasm Bank are base collections; i.e. they are stored for long periods of time and are only used in regeneration processes. Storage is carried out under optimal conditions to maximize seed viability. The procedure followed at the URJC Germplasm Bank was to store seeds in glass tubes in Kilner jars with silica gel. These jars were sealed hermetically and placed in a freezer at -18ºC.

Données de collection

Citations bibliographiques

1. Escudero A, Carnes LF, Pérez-García (1997) Seed germination of gypsophytes and gypsovags in semi-arid central Spain. Journal of Arid Environments 36: 487-497.
2. European Community (1992) Council Directive 92/43/EEC on the conservation of natural habitats and of wild fauna and flora.
3. Fernández-González F (1987) Estudio florístico y fitosociológico del Valle del Paular (Madrid). PhD Thesis, Universidad Complutense de Madrid, Madrid.
4. Ferrandis P, Herranz JM, Copete MA (2005) Caracterización florística y edáfica de las estepas yesosas de Castilla-La Mancha. Investigación Agraria: Sistemas y Recursos Forestales 14: 195-216.
5. García-Romero A, Muñoz-Jiménez J (2010) Modificaciones recientes de la cubierta nival y evolución de la vegetación supraforestal en la Sierra de Guadarrama, España: el Puerto de los Neveros. Cuadernos de Investigación Geográfica nº 36 (2): 107-141.
6. Giménez-Benavides L, Escudero A, Pérez-García F (2005). Seed germination of high mountain Mediterranean species: altitudinal, interpopulation and interannual variability. Ecological Research 20: 433-444. doi: 10.1007/s11284-005-0059-4
7. Giménez-Benavides L (2006) Cambio climático en la alta montaña mediterránea. Ecología reproductiva, potencial adaptativo y viabilidad poblacional de Silene ciliata. PhD Thesis, Móstoles, Madrid.
8. Giménez-Benavides L, Escudero A, Iriondo JM (2007) Reproductive limits of a late-flowering high mountain Mediterranean plant along an elevational climate gradient. New Phytologist 173: 367-382. doi: 10.1111/j.1469-8137.2006.01932.x
9. Gold K, León-Lobos P, Way M (2004) Manual de recolección de semillas de plantas silvestres para conservación a largo plazo y restauración ecológica. Boletín INIA nº 110 (62 p.). Centro Regional de Investigación Intihuasi, Instituto de Investigación Agropecuarias, La Serena, Chile.
10. Gómez-Campo C (1985) The Conservation of Mediterranean Plants: Principles and Problems. In: Gómez-Campo C (Ed) Plant Conservation in the Mediterranean Area. W Junk Publishers, Dordrecht, Holland, 269 pp.
11. Herranz JM, Ferrandis P Copete MA, Martínez-Sánchez JJ (2002) Influencia de la temperatura de incubación sobre la germinación de veintitrés endemismos vegetales ibéricos e iberoafricanos. Invest. Agr.: Prod. Prot. Veg. Vol 17 (2).
12. Martín-Herrero J, Cirujano S, Moreno M, Peris J, Stübing G (2003) La vegetación protegida de Castilla-La Mancha. Descripción, ecología y conservación de los hábitat de protección especial. Dirección General del medio Natural, Conserjería de Medio Ambiente y Junta de Comunidades de Castilla La Mancha, Toledo.
13. Pérez-García F, Durán JM (1989) Germinación de especies endémicas de las regiones mediterránea occidental y macaronésica. Investigación Agraria: Producción y Protección vegetal 4 (1).
14. Prance GT (1997) The Conservation of Botanical Diversity. In: Maxted N, Ford-Lloyd BV, Hawkes JG (Eds) Plant Genetic Conservation. The In Situ Approach: 3-14. Chapman & Hall, London.
15. Rivas Martínez S (1963) Estudio de la vegetación y flora de la Sierra de Guadarrama y Gredos. Anales Jard. Bot. Madrid 21 (2): 5-325.
16. Vielva JA, Granados I, Prieto D (2004) Restauración de ecosistemas de montaña. El Parque Natural de Peñalara. Boletín de la Real Sociedad Española de Historia Natural (sección Geológica) 99 (1-4): 209-216.
17. Vitales D, García-Fernández A, Pellicer J, Vallés J, Santos-Guerra A et al. (2014). Key processes for Cheirolophus (Asteraceae). Diversification on oceanic islands inferred from AFLP data. PLOS One 9(11): e113207. doi:10.1371/journal.pone.0113207

Métadonnées additionnelles