The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils

Antonio José Fernández González1, Antonio José Fernández González2, Katia Cezón2

1 CSIC-Estación Experimental del Zaidín, Granda, Spain; 2 

Corresponding author(s): Antonio José Fernández González (antonio.fernandez@eez.csic.es), Antonio José Fernández González (antonio.fernandez@eez.csic.es)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Fernández González A J (2022): The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils. v1.3. Estación Experimental del Zaidín (CSIC). Dataset/Samplingevent. https://doi.org/10.15470/hhahsj https://doi.org/10.15470/hhahsj

Abstract

Major bacterial agents in a Mediterranean holm-oak forest recovery after a fire

Keywords: Samplingevent, Samplingevent, Metadata, Arthrobacter

Project details

Project title: Project-1.- Análisis de la diversidad procariótica asociada a quercíneas (Quercus ilex y Q. pyrenaica) para la identificación de biomarcadores asociados a la evolución post-incendio y al cambio climático en Sierra Nevada. Project-2.- Identificación de bioindicadores microbianos en la rizosfera de quercíneas (Quercus ilex y Q. pyrenaica) asociados a cambio climático y evolución post-incendio. Project-3.- Adaptación y mejora de la resiliencia del monte mediterráneo frente al cambio climático y los incendios forestales mediante el manejo de microorganismos rizosféricos.
Personnel: Manuel Fernández López, Antonio José Fernández González, José Francisco Cobo-Díaz, Pablo José Villadas
Funding: Department of Innovation, Science and Enterprise of the Autonomous Government of Andalusia; The National Parks Autonomous Body (Ministry of the Environment); the Spanish Ministry of Economy and Competitiveness; and CSIC, including ERDF (European Regional Development Fund).
Study area descriptions/descriptor: Sierra Nevada Natural and National Park, Granada, Spain.

Data published through GBIF: https://ipt.gbif.es/resource?r=zaidin-adn

Taxonomic coverage

Taxonomic ranks
Domain: Bacteria, Archaea
Common names: Bacteria, Archaea

Spatial coverage

General spatial coverage: The soil samples were collected from Lanjarón, Sierra Nevada, Granada, Spain
Undisturbed holm oak forest rhizosphere were collected at 1790 meters above sea level.
Burned holm oak forest rhizosphere were collected at 1566 meters above sea level.
Coordinates: 36°57'36''N and 36°58'12''N Latitude; 3°27'36''W and 3°27'36''W Longitude 

Temporal coverage: April 24, 2008

Natural collections description

Parent collection identifier: No aplica
Collection name: Comunidades rizosféricas de las encinas de Lanjarón tomadas en 2008
Collection identifier: LJN08

Methods

Method step description: The methodological steps were publised in detail in the paper entitled: 
The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils.

With DOI: https://doi.org/10.1038/s41598-017-06112-3
Study extent description: The study area is located in the Sierra Nevada Natural and National Park (SE Spain), where a wildfire in September 2005 burned 3426.74 ha, including 412 ha of evergreen holm oaks (Q. ilex subsp. ballota). Soil samples were collected in the valley of the Lanjarón River where two sites were selected: one in an area directly affected by the wildfire (burned forest containing holm oak trees [BOF]) and a nearby site in evergreen, undisturbed oak forest (UOF)
Sampling description: Three sampling plots were randomly chosen within each study site along transects of 1.0 km length. At the BOF and UOF sites, we sampled the rhizosphere of three trees per plot, each with a diameter of at least 15 cm at breast height and separated by at least 5 m. Sampling took place on April 29, 2008 (3 years after the wildfire). Both sites were on a south-facing steep slope.

The rhizospheric samples were collected from from holm oak rhizosphere soils by following the tree’s main roots until young cork-free roots were found at a distance of less than 50 cm from the trunk. The soil attached to the roots was manually removed and the roots with rhizospheric soil were put into 50 ml Falcon tubes filled with 20 ml of sterilized NaCl 0.8%. After shaking 25 min at 150 rpm and room temperature, the roots were removed and the tubes were centrifuged at 12,850 g for 5 min. The pelleted soil was used to extract environmental DNA. On the other hand, we processed up to 2 kg of soil from each site, sieved through a 2 mm mesh, for physicochemical analysis including soil type, pH, available water, total nitrogen, organic matter, electrical conductivity, etc. All these analyses were carried out with standardized procedures at the Food and Agriculture Laboratory of the Andalusian regional government at Atarfe (Granada, Spain).
Quality control description: In each treatment, 3 healthy trees were taken in 3 independent plots, with more than 1km distance between them to have a representative sample of each site. The DNA from the 3 trees in the same plot was mixed before sequencing to obtain a homogeneous composite sample from each plot. Finally, 3 composite replicates were obtained from each site, BOF as a burn treatment and UOF as a control treatment.

Datasets

Dataset description
Object name: Darwin Core Archive The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: https://ipt.gbif.es/archive.do?r=zaidin-adn
Publication date of data: 2022-09-15
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2020-11-20
Hierarchy level: Dataset