Tree cavities as keystone microhabitats: ecosystem function and conservation in Iberian quercus forests (Fungi)

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如何引用

研究者應依照以下指示引用此資源。:

Tree cavities as keystone microhabitats: ecosystem function and conservation in Iberian quercus forests (Fungi)

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Estación Experimental de Zonas Áridas (CSIC)。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 0d2f3b66-8e5f-4632-8aa9-54fbfdd6d36b。  Estación Experimental de Zonas Áridas (CSIC) 發佈此資源，並經由GBIF Spain同意向GBIF註冊成為資料發佈者。

關鍵字

Samplingevent; metabarcoding; cavity; forest management; biodiversity; Quercus rotundifolia; Observation

聯絡資訊

地理涵蓋範圍

Cabañeros y Monfragüe National parks

分類群涵蓋範圍

N/A

時間涵蓋範圍

計畫資料

無相關描述

參與計畫的人員:

取樣方法

Metabarcoding of wood samples from tree cavities, extracted using an sterilized drill

方法步驟描述:

1. Sampels were obtained using an sterilized drill. DNA was extracted from each sample using the DNeasy PowerSoil Pro kit (Qiagen). The fungal ITS2 region (~330 bp) was amplified using primers ITS3 and ITS4 in a two-step PCR protocol enabling sample multiplexing. Libraries were purified, quantified, pooled in equimolar amounts, and sequenced on a NovaSeq PE250 platform (Illumina), targeting ~4 Gb total output. Adapter removal and primer trimming were performed with Cutadapt. Sequence quality was assessed using FastQC and summarized with MultiQC (Ewels et al. 2016). Reads were quality filtered, denoised, merged, and chimera-checked using DADA2. Amplicon sequence variants were clustered into OTUs at 97 % similarity using DECIPHER to account for multiple ITS copies within fungal genomes as well as intraspecific sequence variation. Taxonomic assignment was performed against the UNITE database using the DADA2 classifier and independently validated using the GBIF Sequence ID tool with BLASTN searches. Final taxonomy was curated combining both approaches, field observations, and phylogenetic analyses.

額外的詮釋資料