Description
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 7,933 records.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Tree cavities as keystone microhabitats: ecosystem function and conservation in Iberian quercus forests (Fungi)
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is Estación Experimental de Zonas Áridas (CSIC). This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: 0d2f3b66-8e5f-4632-8aa9-54fbfdd6d36b. Estación Experimental de Zonas Áridas (CSIC) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Spain.
Keywords
Samplingevent; metabarcoding; cavity; forest management; biodiversity; Quercus rotundifolia; Observation
Contacts
- Originator ●
- Point Of Contact
https://orcid.org/0000-0002-1463-8393
- Originator
- Originator ●
- Point Of Contact
- Point Of Contact
Geographic Coverage
Cabañeros y Monfragüe National parks
| Bounding Coordinates | South West [-90, -180], North East [90, 180] |
|---|
Taxonomic Coverage
N/A
| Kingdom | Fungi |
|---|---|
| Phylum | Blastocladiomycota, Mucoromycota, Sanchytriomycota, Glomeromycota, Ascomycota, Basidiomycota, Chytridiomycota |
| Class | Rhizophydiomycetes, Wallemiomycetes, Tremellomycetes, Atractiellomycetes, Mortierellomycetes, Microbotryomycetes, Glomeromycetes, Malasseziomycetes, Dothideomycetes, Candelariomycetes, Eurotiomycetes, Lecanoromycetes, Chytridiomycetes, Exobasidiomycetes, Sordariomycetes, Saccharomycetes, Sanchytriomycetes, Mucoromycetes, Dacrymycetes, Arthoniomycetes, Agaricomycetes, Cystobasidiomycetes, Ustilaginomycetes, Taphrinomycetes, Pezizomycetes, Xylonomycetes, Leotiomycetes, Orbiliomycetes, Agaricostilbomycetes |
Temporal Coverage
| Start Date / End Date | 2024-05-13 / 2024-05-16 |
|---|
Project Data
No Description available
| Title | Impacto global sobre la formación de cavidades y la diversidad oculta en Parques Mediterráneos |
|---|---|
| Identifier | SPIP2023-029955 |
| Funding | Ministerio para la Transicion Ecologica y el Reto Demografico. Organismo autonomo de Parques Nacionales |
The personnel involved in the project:
Sampling Methods
Metabarcoding of wood samples from tree cavities, extracted using an sterilized drill
| Study Extent | Cabañeros and Monfragüe National Parks. Selected old Quercus rotundifolia stands |
|---|
Method step description:
- Sampels were obtained using an sterilized drill. DNA was extracted from each sample using the DNeasy PowerSoil Pro kit (Qiagen). The fungal ITS2 region (~330 bp) was amplified using primers ITS3 and ITS4 in a two-step PCR protocol enabling sample multiplexing. Libraries were purified, quantified, pooled in equimolar amounts, and sequenced on a NovaSeq PE250 platform (Illumina), targeting ~4 Gb total output. Adapter removal and primer trimming were performed with Cutadapt. Sequence quality was assessed using FastQC and summarized with MultiQC (Ewels et al. 2016). Reads were quality filtered, denoised, merged, and chimera-checked using DADA2. Amplicon sequence variants were clustered into OTUs at 97 % similarity using DECIPHER to account for multiple ITS copies within fungal genomes as well as intraspecific sequence variation. Taxonomic assignment was performed against the UNITE database using the DADA2 classifier and independently validated using the GBIF Sequence ID tool with BLASTN searches. Final taxonomy was curated combining both approaches, field observations, and phylogenetic analyses.
Additional Metadata
| Acknowledgements | |
|---|---|
| Purpose | |
| Maintenance Description | Actualización de los datos o adición de algún campo si es necesario |
| Alternative Identifiers | 10.15470/krdxel |
| 0d2f3b66-8e5f-4632-8aa9-54fbfdd6d36b | |
| https://ipt.gbif.es/resource?r=cavdivfungi |