10.15470/krdxel 0d2f3b66-8e5f-4632-8aa9-54fbfdd6d36b https://ipt.gbif.es/resource?r=cavdivfungi CAVDIVFUNGI Dr. Jesús María Avilés Estacion Experimental de Zonas Aridas (EEZA-CSIC)

La Cañada de San Urbano Murcia 04120 ES

javiles@eeza.csic.es http://www.eeza.csic.es/personales/javiles.aspx 0000-0002-1463-8393 Dr. Ibai Olariaga Ibarguren Universidad Rey Juan Carlos

Móstoles Madrid 28933 ES

ibai.olariaga@urjc.es 0000-0002-0334-7750 María Prieto Álvaro Universidad Rey Juan Carlos

Móstoles Madrid 28933 ES

maria.prieto@urjc.es 0000-0002-1692-9821 2026-10-13 eng This dataset contains records based on ITS metabarcoding data generated from drilled wood samples collected from holm oak (Quercus ilex) cavities in two Iberian Mediterranean forests (Cabañeros and Monfragüe National Parks). The goal of the study is to characterize fungal communities associated with cavity formation and wood decay and to improve understanding of the biological processes contributing to cavity development in oak ecosystems. ITS sequences were curated by removing of chimeric and non-fungal sequences. This research was supported by grant the National Parks Project (SPIP2023-029955) with Jesus M. Avilés as principal investigator and Maria Prieto & Ibai Olariaga as collaborators in the fungal part of the project. Samplingevent metabarcoding cavity forest management biodiversity Quercus rotundifolia GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml Observation GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License. Creative Commons Attribution Non Commercial 4.0 International https://spdx.org/licenses/CC-BY-NC-4.0.html CC-BY-NC-4.0 https://ipt.gbif.es/archive.do?r=cavdivfungi Cabañeros y Monfragüe National parks -180 180 90 -90 2024-05-13 2024-05-16 N/A phylum Blastocladiomycota class Rhizophydiomycetes class Wallemiomycetes class Tremellomycetes kingdom Fungi class Atractiellomycetes phylum Mucoromycota class Mortierellomycetes class Microbotryomycetes phylum Sanchytriomycota class Glomeromycetes class Malasseziomycetes class Dothideomycetes phylum Glomeromycota class Candelariomycetes class Eurotiomycetes class Lecanoromycetes class Chytridiomycetes class Exobasidiomycetes class Sordariomycetes class Saccharomycetes phylum Ascomycota class Sanchytriomycetes class Mucoromycetes class Dacrymycetes class Arthoniomycetes class Agaricomycetes class Cystobasidiomycetes class Ustilaginomycetes class Taphrinomycetes phylum Basidiomycota class Pezizomycetes phylum Chytridiomycota class Xylonomycetes class Leotiomycetes class Orbiliomycetes class Agaricostilbomycetes The purpose of this project is to make available for biodiversity data users the occurrences derived from the project This research was partly funded by the Research Project Grants Program in National Parks (Autonomous Agency for National Parks, OAPN) through grant SPIP2023-02995 awarded to the authors.We are deeply grateful to the private landowners across the multiple sampling locations who, over the past three decades in both private estates and national and regional parks, generously granted access for fieldwork. In particular, we thank the owners and managers of the Navacalera and Las Corchuelas estates for facilitating surveys. We also acknowledge the logistical support of park rangers, visitor services staff, and national park directors for their commitment to this project. Special thanks are extended to María Jesús Sánchez Soler (Director of Cabañeros National Park) for her continuous support and availability. We also thank Pablo Beltrán López for assistance with bioinformatic analyses of fungal datasets, and Chano Robles and Carlos Ciudad for their essential field support in Quercus pyrenaica forests in León. Actualización de los datos o adición de algún campo si es necesario asNeeded Dr. Jesús María Avilés Estacion Experimental de Zonas Aridas (EEZA-CSIC)

La Cañada de San Urbano Murcia 04120 ES

javiles@eeza.csic.es http://www.eeza.csic.es/personales/javiles.aspx 0000-0002-1463-8393 Dr. Ibai Olariaga Universidad Rey Juan Carlos

Móstoles Madrid 28933 ES

653731811 ibai.olariaga@urjc.es 0000-0002-0334-7750 Dra. María Prieto Álvaro Universidad Rey Juan Carlos

Móstoles Biología 28933 ES

maria.prieto@urjc.es 0000-0002-1692-9821 Sampels were obtained using an sterilized drill. DNA was extracted from each sample using the DNeasy PowerSoil Pro kit (Qiagen). The fungal ITS2 region (~330 bp) was amplified using primers ITS3 and ITS4 in a two-step PCR protocol enabling sample multiplexing. Libraries were purified, quantified, pooled in equimolar amounts, and sequenced on a NovaSeq PE250 platform (Illumina), targeting ~4 Gb total output. Adapter removal and primer trimming were performed with Cutadapt. Sequence quality was assessed using FastQC and summarized with MultiQC (Ewels et al. 2016). Reads were quality filtered, denoised, merged, and chimera-checked using DADA2. Amplicon sequence variants were clustered into OTUs at 97 % similarity using DECIPHER to account for multiple ITS copies within fungal genomes as well as intraspecific sequence variation. Taxonomic assignment was performed against the UNITE database using the DADA2 classifier and independently validated using the GBIF Sequence ID tool with BLASTN searches. Final taxonomy was curated combining both approaches, field observations, and phylogenetic analyses. Cabañeros and Monfragüe National Parks. Selected old Quercus rotundifolia stands Metabarcoding of wood samples from tree cavities, extracted using an sterilized drill Jesús M. Avilés Regodón 0000-0002-1463-8393 author Ministerio para la Transicion Ecologica y el Reto Demografico. Organismo autonomo de Parques Nacionales 2026-10-07T19:01:24.388+02:00 dataset Tree cavities as keystone microhabitats: ecosystem function and conservation in Iberian quercus forests (Fungi) 0d2f3b66-8e5f-4632-8aa9-54fbfdd6d36b/v2.xml